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重组抗体

- CD31 Antibody
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凯发K8旗舰生物抗体平台现已拥有4万多种抗体(含单抗和多抗),包括人类常用抗体,模式生物类相关抗体、小分子抗体、二抗、标签抗体及内参抗体等。并以每年4000种的速度在持续增长。
凯发K8旗舰生物拥有专业的质控团队,配备先进实验仪器,现已成功地建立了ChIP平台,因此我们的抗体不仅能给予Biotin、FITC、HRP等标记,大部分抗体都能进行ELISA、WB、IHC、IF、IP、ChIP等应用验证。
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CD31 Antibody
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货号:HYK-200278
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规格:
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图片:
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Western Blot
Positive WB detected in:THP-1 whole cell lysate
All lanes:CD31 antibody at 0.95μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 125 KDa
Observed band size: 125 KDa -
IHC image of CSB-RA017767A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Overlay histogram showing Jurkat cells stained with CSB-RA017767A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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产品详情
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Uniprot No.:P16284
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基因名:PECAM1
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别名:Adhesion molecule antibody; CD31 antibody; CD31 antigen antibody; CD31 EndoCAM antibody; EndoCAM antibody; FLJ34100 antibody; FLJ58394 antibody; GPIIA antibody; GPIIA' antibody; PECA1 antibody; PECA1_HUMAN antibody; Pecam 1 antibody; PECAM 1 CD31 EndoCAM antibody; PECAM antibody; PECAM-1 antibody; Pecam1 antibody; Platelet and endothelial cell adhesion molecule 1 antibody; Platelet endothelial cell adhesion molecule antibody; Platelet/endothelial cell adhesion molecule 1 antibody
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反应种属:Human
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免疫原:A synthesized peptide
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免疫原种属:Homo sapiens (Human)
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标记方式:Non-conjugated
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克隆类型:Monoclonal
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抗体亚型:Rabbit IgG
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纯化方式:Affinity-chromatography
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浓度:It differs from different batches. Please contact us to confirm it.
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保存缓冲液:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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产品给予形式:Liquid
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应用范围:ELISA, WB, IHC, FC
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推荐稀释比:
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:500 -
Protocols:ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
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储存条件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or , please kindly consult your local distributors for specific delivery time.
CD28 Antibody
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CD16 Antibody
CD13 Antibody
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