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语言:Eng
重组抗体

- ACTA1 Antibody
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凯发K8旗舰生物抗体平台现已拥有4万多种抗体(含单抗和多抗),包括人类常用抗体,模式生物类相关抗体、小分子抗体、二抗、标签抗体及内参抗体等。并以每年4000种的速度在持续增长。
凯发K8旗舰生物拥有专业的质控团队,配备先进实验仪器,现已成功地建立了ChIP平台,因此我们的抗体不仅能给予Biotin、FITC、HRP等标记,大部分抗体都能进行ELISA、WB、IHC、IF、IP、ChIP等应用验证。
我们致力于为所有用户给予高亲和力、高特异性的优质抗体,欢迎来电咨询。
ACTA1 Antibody
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货号:HYK-200292
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规格:
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图片:
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Western Blot
Positive WB detected in:Hela whole cell lysate,A549 whole cell lysate,Raw264.7 whole cell lysate,NIH/3T3 whole cell lysate,HepG2 whole cell lysate,Rat brain tissue,Rat heart tissue
All lanes:Actin antibody at 0.95μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 KDa
Observed band size: 42 KDa
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IHC image of CSB-RA001205A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of HepG2 cells with CSB-RA001205A0HU at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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产品详情
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Uniprot No.:P68133
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基因名:ACTA1
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别名:a actin antibody; ACTA antibody; ACTA1 antibody; ACTA2 antibody; ACTC antibody; ACTC1 antibody; Actin antibody; ACTS_HUMAN antibody; ACTSA antibody; Alpha 2 actin antibody; alpha skeletal muscle antibody; Alpha-actin-1 antibody; Cardiac muscle alpha actin 1 antibody; Skeletal muscle alpha actin 1 antibody
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反应种属:Human
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免疫原:A synthesized peptide
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免疫原种属:Homo sapiens (Human)
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标记方式:Non-conjugated
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克隆类型:Monoclonal
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抗体亚型:Rabbit IgG
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纯化方式:Affinity-chromatography
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浓度:It differs from different batches. Please contact us to confirm it.
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保存缓冲液:Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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产品给予形式:Liquid
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应用范围:ELISA, WB, IHC, IF, FC
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推荐稀释比:
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:500 IF 1:30-1:200 -
Protocols:ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
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储存条件:Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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货期:Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Histone H2B type 1-K Antibody
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在线询价
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